FAS anticorps

N° du produit ABIN2689461

L’anticorps Hamster arménien Monoclonal anti-FAS a été validé pour FACS, IP et CyTox. Il convient pour détecter FAS dans des échantillons de Souris. Il y a 5+ publications disponibles.

Aperçu rapide pour FAS anticorps (ABIN2689461)

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp FAS est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Cytotoxicity Test (CyTox)

Clone: Jo2

Détail du produit anti-FAS anticorps

Marque: BD Pharmingen™

Attributs du produit: Fas antigen, CD95, is a 45 kDa cell-surface protein which can mediate apoptosis. It belongs to the TNF (tumor necrosis factor)/NGF receptor family. Expression of Fas has been described in the thymus, liver, heart, lung and ovary. Fas plays an important role in the apoptotic process that takes place during development. Monoclonal antibodies recognizing Fas such as Jo2 have cytolytic activity on cells expressing Fas. The cell death stimulated by Fas antibodies is characteristic of apoptosis and suggests that the lethal effects are a result of interaction of antibody with a functional Fas antigen as opposed to complement-mediated lysis. The Jo2 antibody recognizes mouse Fas. The Jo2 antibody shows cytolytic activity against cell lines expressing mouse Fas by inducing apoptosis. Intraperitoneal injections of Jo2 mAb have been shown to kill mice and induce apoptotic hepatocyte death. Jo2 mAb immunoprecipitates mouse Fas as a 45 kDa band from W4 cells. W4 cells are WR19L mouse lymphoma cells transformed with mouse Fas. The difference between the observed MW of Fas and that deduced from its amino acid sequence (Mr 34,971) may be due to glycosylation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of Fas on mouse thymocytes analyzed by flow cytometry. Thymocytes from a C57BL/6 mouse were incubated with either Jo2, followed by a FITC-conjugated mouse anti-hamster second step (filled histogram, or with second step alone (open histogram).

BD Pharmingen™ Purified Hamster Anti-Mouse CD95 - Purified - Clone Jo2 - Isotype Armenian Hamster IgG2, λ2 - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: WR19L mouse lymphoma cells transformed with recombinant mouse Fas

Isotype: IgG2 lambda

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-FAS (ABIN2689461)

Ogasawara, Suda, Nagata: "Selective apoptosis of CD4+CD8+ thymocytes by the anti-Fas antibody." dans: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 485-91, (1995) (PubMed).

Yang, Merćep, Ware, Ashwell: "Fas and activation-induced Fas ligand mediate apoptosis of T cell hybridomas: inhibition of Fas ligand expression by retinoic acid and glucocorticoids." dans: The Journal of experimental medicine, Vol. 181, Issue 5, pp. 1673-82, (1995) (PubMed).

Hiromatsu, Aoki, Makino, Matsumoto, Mizuochi, Gotoh, Nomoto, Ogasawara, Nagata, Yoshikai: "Increased Fas antigen expression in murine retrovirus-induced immunodeficiency syndrome, MAIDS." dans: European journal of immunology, Vol. 24, Issue 10, pp. 2446-51, (1994) (PubMed).

Kägi, Vignaux, Ledermann, Bürki, Depraetere, Nagata, Hengartner, Golstein: "Fas and perforin pathways as major mechanisms of T cell-mediated cytotoxicity." dans: Science (New York, N.Y.), Vol. 265, Issue 5171, pp. 528-30, (1994) (PubMed).

Ogasawara, Watanabe-Fukunaga, Adachi, Matsuzawa, Kasugai, Kitamura, Itoh, Suda, Nagata: "Lethal effect of the anti-Fas antibody in mice." dans: Nature, Vol. 364, Issue 6440, pp. 806-9, (1993) (PubMed).

Détails sur FAS

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Autre désignation: CD95

Sujet: Synonyms: Fas/APO-1

Poids moléculaire: 45 kDaTarget Molecular Weight: Aqueous buffered solution containing ≤0.09% sodium azide.

Pathways: Signalisation p53, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity